Journal: Oncotarget
Article Title: CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis
doi: 10.18632/oncotarget.9899
Figure Lengend Snippet: ( A – B ) Dose-dependent recruitment of F4/80 + macrophages by TSP1 measured by Transwell assay. Shown are representative images of DAPI-stained macrophages (Scar bar, 50 μm) in the outer chamber (A) and numbers of macrophages per 100× microscopic filed (B). ( C ) TSP1 mRNA levels in 24-hr cultured tumor cells from WT and CD47 −/− mice ( n = 4/group). ( D – E ) Recruitment of WT macrophages by cultured tumor cells from WT mice, CD47-deficient mice without (CD47 −/− ) or with (CD47 −/− + TSP1) recombinant mTSP1 (2.2 nM). Shown are representative flow cytometry profiles (D) and percentages (E) of F4/80 + macrophages in the outer chambers. ( F – G ) Recruitment of WT (WT MΦ) and CD47-deficient (CD47 −/− MΦ) macrophages by cultured tumor cells from WT or CD47-deficient mice. Shown are representative flow cytometry profiles (F) and percentages (G) of F4/80 + macrophages in the outer chamber of the transwell plate. Data are mean ± SDs. * P < 0.05, ** P < 0.01; *** P < 0.001.
Article Snippet: Where indicated, recombinant mouse TSP1 (R&D Systems) was added into the outer chamber.
Techniques: Transwell Assay, Staining, Cell Culture, Recombinant, Flow Cytometry